anti human adrp  (Proteintech)

Journal: Autoimmunity

Article Title: Anti-perilipin-1 autoantibodies in autoimmune Addison's disease and related endocrine disorders.

doi: 10.1080/08916934.2025.2461703

Figure Lengend Snippet: Figure 1. Anti-PLIN1-autoAbs in patients with autoimmune endocrine diseases. Anti-PLIN 1-autoAbs indices in APS-1, AAD/APS-2, IAD, GD, T1D, POI and blood donor groups. Mean index values for each patient group are marked by horizontal lines. Threshold for positivity (horizontal dashed red line) has been calculated for the PLIN1 autoantigen based on the mean of indices of blood donors + 3SD (index ≤122). Thirteen dominant negative mutations in AIRE patients (gray dots) are included in the APS-1 patient subgroup. APS-1: autoimmune polyendocrine syndrome type 1; AAD: autoimmune Addison’s disease; APS-2: autoimmune poly endocrine syndrome type 2; IAD: idiopathic Addison’s disease; GD: graves’ disease; T1D: type 1 diabetes; POI: primary ovarian insufficiency; SS: Sjögren’s syndrome.

Article Snippet: For coating of wells, 50 μL recombinant PLIN1 protein (Cat: TP306292, Origene) at 2.0 μg/ mL in 1X PBS (Cat: 524650, EMD Millipore,) was incubated overnight at 4 °C in a 96-well ELISA plate (Nunc MaxiSorpTM Flat-bottom, Cat: 439454, Thermo Fisher Scientific).

Techniques: Dominant Negative Mutation

Journal: Autoimmunity

Article Title: Anti-perilipin-1 autoantibodies in autoimmune Addison's disease and related endocrine disorders.

doi: 10.1080/08916934.2025.2461703

Figure Lengend Snippet: Figure 2. A longitudinal study of PLIN1-autoAbs for an APS-1 patient (patient #27, Table 2). PLIN1 indices of five serial samples from the same patient at five different time points in the period 2009–2021, and 21OH autoAbs indices plotted at the same time spanning. The thresholds for positivity to PLIN1-autoAbs and 21OH autoAbs are shown with horizontal dashed red and black lines, being 122, and 100 respectively.

Article Snippet: For coating of wells, 50 μL recombinant PLIN1 protein (Cat: TP306292, Origene) at 2.0 μg/ mL in 1X PBS (Cat: 524650, EMD Millipore,) was incubated overnight at 4 °C in a 96-well ELISA plate (Nunc MaxiSorpTM Flat-bottom, Cat: 439454, Thermo Fisher Scientific).

Techniques:

Journal: Autoimmunity

Article Title: Anti-perilipin-1 autoantibodies in autoimmune Addison's disease and related endocrine disorders.

doi: 10.1080/08916934.2025.2461703

Figure Lengend Snippet: Figure 3. Analysis of anti-PLIN1 IgM autoantibodies using ELISA. Sera samples of anti-PLIN1-autoAbs positive patients in radioimmunoassay were used to detect frequencies of IgM PLIN1-autoAbs through ELISA. (A) Frequency of anti-PLIN1 IgM autoAbs compared to control. (B) Serial sera samples from one patient with APS-1 (patient #27) analyzed for PLIN1- IgM autoAbs in ELISA. A commercial monoclonal antibody against PLIN1 was used as positive control. In (A) the blue dot depicts one blood donor scoring PLIN1 autoAbs borderline positive in RIA. In (A and B) the violet dot corresponds to the identical sample that has also been plotted in the PLIN+ in both the RIA and longitudinal groups.

Article Snippet: For coating of wells, 50 μL recombinant PLIN1 protein (Cat: TP306292, Origene) at 2.0 μg/ mL in 1X PBS (Cat: 524650, EMD Millipore,) was incubated overnight at 4 °C in a 96-well ELISA plate (Nunc MaxiSorpTM Flat-bottom, Cat: 439454, Thermo Fisher Scientific).

Techniques: Enzyme-linked Immunosorbent Assay, RIA Assay, Control, Positive Control

Journal: International journal of molecular sciences

Article Title: Myocardial Fibrosis and Steatosis in Patients with Aortic Stenosis: Roles of Myostatin and Ceramides.

doi: 10.3390/ijms242115508

Figure Lengend Snippet: Figure 1. Histological and immunohistochemical analysis of LV tissue sections. (A): Interstitial fibrosis in LV sections stained with Masson’s trichrome, where fibrous collagen is shown in blue and myocytes in red (scale bar = 100 µm). (B): Myocardial cell apoptosis in LV sections assessed with TUNEL, which highlights nuclei of apoptotic cells in blue or black, and nuclei of non-apoptotic cells in red (scale bar = 25 µm). (C): PLIN5 immunohistochemical staining, indicative of ventricular lipid infiltration, with PLIN5 positive cardiomyocytes in brown (scale bar = 25 µm). (D): Immunohistochemical ceramide staining of LV cardiomyocytes (scale bar = 50 µm). (E): Immunohistochemical staining for myostatin with myostatin highlighted in brown and the nuclei in blue (scale bar = 100 µm). LV = left ventriculum. PLIN = perilipin.

Article Snippet: The following primary antibodies were used for the IHC analysis: rabbit anti-human perilipin-1 (PLIN1) (1:150; cat. n◦: 9349; Cell Signaling, Danvers, MA, USA) to detect adipocytes, rabbit antihuman perilipin-5 (PLIN5) (1:1000; cat. n◦: PA1-46215, Thermo Fisher Scientific, Waltham, MA, USA) to mark lipid droplets inside cardiomyocytes, and rabbit anti-human myostatin (1:25; cat. n◦: ab71808, Abcam, Cambridge, UK).

Techniques: Immunohistochemical staining, Staining, TUNEL Assay

Journal: International journal of molecular sciences

Article Title: Myocardial Fibrosis and Steatosis in Patients with Aortic Stenosis: Roles of Myostatin and Ceramides.

doi: 10.3390/ijms242115508

Figure Lengend Snippet: Figure 3. Histological characteristics of the LVs of AS patients, according to the echocardiographic values of mean gradient (MG) and peak gradient (PG). Specific histological features of LVs expressed as means ± SD according to tertiles of MG and PG. The overall significance across groups is also indicated. Significance levels for individual comparisons are identified as * p < 0.05, ** p < 0.01, and *** p < 0.001 for comparison of low and medium tertiles vs. high tertile; ns: no significant difference. LV = left ventriculum. AS = aortic stenosis. SD = standard deviation. PLIN = perilipin.

Article Snippet: The following primary antibodies were used for the IHC analysis: rabbit anti-human perilipin-1 (PLIN1) (1:150; cat. n◦: 9349; Cell Signaling, Danvers, MA, USA) to detect adipocytes, rabbit antihuman perilipin-5 (PLIN5) (1:1000; cat. n◦: PA1-46215, Thermo Fisher Scientific, Waltham, MA, USA) to mark lipid droplets inside cardiomyocytes, and rabbit anti-human myostatin (1:25; cat. n◦: ab71808, Abcam, Cambridge, UK).

Techniques: Comparison, Standard Deviation